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Do my capstone presentation template usmc capstone concept reportaze brygidy kmiecik wieck ´╗┐to our next speaker who is I don't Anna pal from the University of Missouri and Aaron's going to be talking about mining Serbian genetic diversity to identify genetic markers and physiological mechanisms underlying carbon isotope discrimination good morning to all of you I thank the organizing committee chair and culture are giving me this wonderful opportunity with died I would like to start with the paseos equation where the granny is equal to amount of water used in to what is efficiency into harvest index as you know that the water is efficiency is one of the difficult flight from SL in the field so we used carbon Isaac of discrimination as a surrogate to Messer buttress efficiency in our soybean study achoo over knowledge genetics of CID in soybean is an activist so actually be screened large amount of swiping germplasm in order to do g was steady to identify putative lousy and also another reason is to identify extreme genotypes for making crosses and also to do other physiological studies so as you can see from this graph the number of genotypes is on the x axis of the soybean by Omar series on the way exists you see a good variability for the cid in our collection so we actually conducted that the g wats analysis over for environment with implications and we identified about 39 snip markers tagging 21 / getty browsing and this is the promos on map which was a protective low self or cid where you can see the chromosomal position on the x-axis on the chromosome on the y axis and the dots that considered the line from one to 20 are nothing but the number of snips which is used about twenty twelve thousand states which we used and the small green circle blue on the red or nothing but the snitch that are identified based on the different analysis method we did report previously identified you deal with the purple squares and the pink and purple triangles though one of the important thing which we need to see in this figure is the genetic loci which are been identified by more than one analysis method which are being highlighted with the yellow circles over here so with this we can rightly say that the good variability for cid existed in the soybean zoom plasm that we study and how it can be rightly used in the chivas approach to identify genetic closely once we identified the extreme of a next interest was to see how the soybean CID is related with the water use efficiency so in order to find this we actually done both field and greenhouse experiment how the field experiments was done in that route simulator or rainout shelter that we had at a platform at University of Missouri and the green notes we used was last Landry nose in University of Missouri as well so we actually examine that contrasting genotypes for low cid and high sierra imposing different water availability treatments and palpated their water use and later we determined the carbon isotope signatures for all the genotypes as we selected the cid as we selected the genotypes based on the cid here you can see how they perform I mean these two lines which are with the red bars are the lines which are selected for the losi ad which will be sort the higher water use efficiency and these two lines are for the higher cid which would know what Reese efficiency and the graph on the right with source which is for like a check for us to see whether what we have done is right so these genotypes underwater limited condition so higher what we see efficiency which is what important for us we were thinking about whether we could still see some differences using some cairo composers so we actually determined that i put to thermal imaging for canopy temperature depressant and what we found out was the no see ad genotypes had lower canopy temperature do present and that's the high cid genotypes had higher CTD so the correlation between the CPD and the CID was positive whereas the correlation between ctd and what insufficiency was negative and this result shows as the thermal images can be successfully used in order to differentiate the genotypes for what is deficiency these results are coming from the pot experiment which was conducted in the hoop house value where we were interested to see how this extreme genotypes respond to the photosynthesis instrumetal context as in the x-axis you see the time of the day and the and the photosynthesis on the y-axis on the left over here where you see the High Sierra 0 types of the world water condition it soared higher photosynthesis and same the hi-c ad also had the highest or metal conductance so when when when we see when we say that that is highest for metal conducts and absolutely higher fans brazen as you can see in the figure over here we did observe that as well I mean the pants prisoners actually calculated by placing the pots on the scale and measuring that man water loss for every five minutes and as you can see here the high CID genotypes had higher transpiration and when we try to offer age them and the sum of three days transportation is shown over here but the high CDs genotypes or headlamp reason but in conclusion we can say that the High Sierra genotypes have high hopes for metal conductance photosynthetic rate and transpiration rate so as you can see from this with cells you may be assuming whether there is rather low see ad genotypes are more resistant to yield I mean that is what we actually done some premiere the experiment and high I have some results over here with where you can see we'd have done to type of experiments one using the Pantene products and on one you're using electrical to us though the whole the whole cid on the exact is over here on the yield for the panda protection on the y-axis but you see a good relationship between this but i think this is not our interest because when you select for a higher yield over here then you will be selecting for the higher see ad actually we need to select for the low cid with a higher real so I mean in that in the figure on the right where you can see with iolite forgive us but the for alive healthy was which were activated in four different locations we see a good negative relationship over here where when you see here for the low CID we are selecting a higher yield this is worse is more interesting for us and to answer the question whether the high yield is associated with the higher you hire watery sufficiency of course yes based on their life healthy worst of course the difference exists between the cultivars on the PA and one of the reason for this would be that the VAR from a jump awesome bank are they are always go yielding whether their late 20 verse that is a good yield so that we need to study this further and another reason might be also the effect of the environmental condition where these few experiments was not conducted in the same year they have been conducted in the different here so with that I would like to conclude that the genetic variability for the CID exist in the soybean genotypes and the genotypes with the lower cid exhibited higher water efficiency in both in our greenhouse studies and field studies and thermal imaging can be useful to identify the genotypes differing for the water its efficiency so the superior water its efficiency may be leveraged to develop drought tolerant are diverse so our future what would involve conforming this genetic markers for use in selecting the genotype or higher water efficiency and we would like to further know whether is there any yield finality or improvement with selection for is there is any room for improvement for selection with participation seals why bean so we are more interested also to seen on stomatal limitations such as miss opal conductance a density of stomata and also rubisco partitioning may play an important role so we are trying to explore this further and we are also interested to see how the genotype entry and Ron metal in fact sense works for our extreme saw CID genotypes with that I would like to acknowledge my supervisor Felix preaching and most of the fieldwork screening has been done by seeing on Villar you and the greenhouse and the growth chamber experiments was done by brad nailer and Alvaro and we did hireable operators from usda-ars and university of arkansas name but um can we take questions at the end mr. together yeah thanks top research topics in networking Wagner College.

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