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Capstone project comments online write for me capstone drug testing pixeom seminar report ´╗┐so for pickles it's the easiest preparation type to do and I always teach my students starting with this method because it allows us to focus all of our attention on cats getting data filling out tags and taking tissue without also getting into all the complicated things of making skins and skeletons so this isn't a bad place to start with students and what's interesting is it will take you about three minutes to prepare the specimen whereas it'll take you about three hours to get through teaching students how to fill out tags take the data and take the tissue specimen but so once you've done all that once you've got all your data and you've added a tag now unlike with skins well you're gonna use special fluid tags for fluid specimens the usual paper tags that the ideal tag for a skin is going to be a different kind of paper but so there's fluid tags that are special that can go into alcohol or formalin and still hold the ink you do not want to use ethanol that will run with with any of the fluids that you don't want to preserve in so you can use these pigment pens over here or you can use pencil we usually use pigment pens and the other thing is because part of the curatorial process is usually that you assign a specimen a preparator x' number because you just don't know what the outcome is going to be with asbestos world the preparation that has to travel through before it can be curated in the collection but with a pickle by the time you get to this point you're just minutes away from being done and so we assign our cure our catalog number the official final catalog number as prior to pickling it and we write that on the tag so that it goes in there and we are done yeah and jump in with anything guys because I you know I actually don't have a lot of feel about what other people do so at this point I typically would have I don't do sexing I might I always take a pictorial sample tissue sample sometimes I do a liver sample but that's usually kind of all I do then I teach my students I teach them what I call the nine point injection and nine point injection is definitely overkill for something like a a goldfinch or a small passerine because basically formalin is going to fix by permeating the tissue pretty quickly but it's just good to have a model in your head for where you would need to inject if you needed to get your fluid deeper into the specimen the whole idea is to introduce the fluid so that preservation occurs before degradation typically people I think are still preparing using formalin as their primary fixative they're you know I've talked to some people because they're the problem with formalin is that it fixes DNA so you lose the ability to extract certain histological things from formalin-fixed festival and formalin can be a nasty chemical to work with so so I'm curious does anybody use ethanol as their primary preservative if you were really do different types of studies at all would be fine but again it's not a fixed it because formalin actually puts a protein coat across to cells and links those and that's why it's hard to break but so ethanol leaves you vulnerable to future degradation but yeah it has its own and Nikita both is preserving it and immediately but we're all from doing camp we have our tags ready but we're have all the data ready to go before we even kill a bird Suzette bird is dead we're injecting that within three or four minutes to maximally preserve because inside what especially if you shot at birth that's the other thing degradation can begin immediately likewise with roadkill oh I guess the other thing I should say about this this is just informative is that people a lot of times say oh look at the skin is all shot up it's not gonna make a very good skin the skeletons all shot up let's let's just make a pickle and this is the wrong idea do not do that because I has a person who's used pickles if you can't make a skin out of it if you can't put a skeleton together you're not gonna be able to get anything skeletal muscular layout it's going to be a big mess you might as well actually the best thing to do with those is to struggle and make a skin and actually you want the most pristine specimens possible for your pickles to make sure you've got good skeletal muscular good neural good anatomical inside the specimen okay but the actual preparation all that being said is the nine point injection which is the nine points are left right for limb left right hind limb left right pectoral - in the gut and then don't forget about the head so now here's where we'll actually do the demo you're just going to use a syringe and a needle always teach your students to poke away from themselves and not towards themselves i I've found that this is a great habit to develop is to hold on to the limb put the torso of the bird away from you and push into the bird with the tip of your needle slide it under the skin add a nice slanting angle so you're not jabbing it through and then you can inject a little bit of fluid I don't know that's pretty subtle to keep you can you can see that usually you can see a ballooning of the being in around where you put the fluid and you only need a couple you know a CC a half a CC in a small password is overkill in a bird this size I'll give a couple of cc's of fluid in each injection so now I'm going to do the other side so this is two of nine again just sliding it into the muscle introducing that preservative into the thicker tissue parts there's swing Wormsley now I do first of all the inside of the wing here has got a nice bald patch with a lot of muscles so this is a perfect place on the bird and likewise on the inside of the upper thigh is a great place that's usually kind of bald so you can see what's going on it's awfully dark isn't it like you can see something okay there's not a whole lot to see yeah because remember that especially formalin-fixed any kind of that molecular stuff that you might have wished you could have camped it's gonna be lost so there's there's no you don't need to use a new needle no you're not doing molecular analyses with these kinds of specimens you're doing histological or morphological no no that's a good question if you went for ethanol maybe but again I mean you take a tissue sample prior if you were doing ethanol that's right that's right okay so I've done both legs I'll do the pectoral region this is a nestling so not a whole lot of pectoral to get into also if you've taken a tissue sample the pectoral the one of your muscles is open and therefore is going to be totally exposed to food anyways then I usually do one injection high in the gut like in the heart lung area one low in the gut to the you know the intestinal area whoops and if you hit the stomach nothing's going to happen okay and then the last one is in the head the region of the head and it's hard to put a needle in between the skin and the skull I usually get right behind the jaw there's a little bit of muscle there and you can just introduce a little fluid there hopefully you saw that kind of ballooned up like that yes yes yeah and that's kind of it your visit that is specimen preparation then I usually try and put it in sort of the fetal position before you put it in so it doesn't have legs and stuff sticking out to knock into other specimens and then you can just immerse it in the whole jar of formalin in this case just to reiterate on that a nice dry festive is a whole lot of hair and you have to introduce not just water but the tiny little film of soap in there so that the water can saturate so that does not sit as much as I can and use it very sparingly you enterprise risk management training in dubai Eastman School of Music.

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